SOX3 Antibody AF2569 from R&D Systems, a Bio-Techne Brand

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Cell reports
February 16 2021

Fig 1: Signaling pathways that regulate transitions in cell state among mouse SSCs and progenitors(A–C) Phosphorylated MAPK (phospho-MAPK) (A), phosphorylated AKT (phospho-AKT) (B), and p-RPS6 (C) levels in isolated seminiferous tubule cells from adult Id4-Egfp mice gated for quiescent SSCs (ID4-EGFPBright/SOX3low/Ki67low, orange), activated SSCs (ID4-EGFPBright/SOX3low/Ki67high, green), and early progenitors (ID4-EGFPBright/SOX3high, red) compared with unstained negative control cells (gray). Gating controls are in Figure S3H.(D) Quantification of flow cytometry from (A)–(C) (n = 3 adult Id4-Egfp mice). Dot size, proportion of undifferentiated spermatogonia; color, percentage that are marker positive.(E) WM-IIF of PLZF (green, spermatogonia), p-RPS6 (red, mTORC1 activity), SOX3 (white, early/late progenitors), and Ki67 (blue, proliferation) in adult C57BL/6 mice. Arrowhead, quiescent SSCs (PLZF+/SOX3-/Ki67-); circle, early progenitors (PLZF+/SOX3+/Ki67+). Scale bar, 20 µm.(F) Quantification of p-RPS6 levels in quiescent SSCs (ID4-EGFPBright/SOX3low/Ki67low), activated SSCs (ID4-EGFPBright/SOX3low/Ki67high), and early progenitors (ID4-EGFPBright/SOX3high) in untreated and rapamycin-treated adult Id4-Egfp mice (n = 3 adult Id4-Egfp mice). Dot size, proportion of undifferentiated spermatogonia; color, percentage marker positive.(G) Proportion of SSCs (KIT-/PLZFlow/SOX3low/RAR?low), early progenitors (KIT-/PLZFlow/SOX3high/RAR?low), and late progenitors (KIT-/PLZFhigh/SOX3high/RAR?high) in untreated and rapamycin-treated adult C57BL/6 mice (see controls in Figure S3J). Data are mean ± SEM. Two-tailed Student’s t test (*p < 0.05, **p < 0.01, and ***p < 0.001).(H) Quantification of flow cytometry analysis of Ki67 levels in untreated (n = 3) and rapamycin-treated (n = 3) adult C57BL/6 mice gated for SSCs (KIT-/PLZFlow/SOX3low/RAR?low), early progenitors (KIT-/PLZFlow/SOX3high/RAR?low), and late progenitors (KIT-/PLZFhigh/SOX3high/RAR?high). Gating controls are in Figure S3J. Data are mean ± SEM. Two-tailed Student’s t test (*p < 0.05 and **p < 0.01).
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Cell reports
February 16 2021

Fig 2: Cell-cycle-activated and quiescent SSCs are present throughout the cycle of the seminiferous epithelium in vivo(A) Whole-mount immunofluorescence (WM-IIF) of SOX3 (white) and Ki67 (red), together with ID4-EGFP epifluorescence (green) in seminiferous tubules from adult Id4-Egfp mice. Scale bar, 20 µm. Arrowhead, quiescent SSCs (ID4-EGFPBright/SOX3-/Ki67-); asterisk, late progenitors (ID4-EGFPDim/SOX3+/Ki67-).(B) Flow cytometry analysis of adult Id4-Egfp mouse seminiferous tubule cells sequentially gated for SSC-enriched CD9Bright/ID4-EGFPBright spermatogonia (left) and SOX3 and RAR? staining (right). Figure S3H shows negative controls.(C) Quadrant statistics from the right panel of (B). Data are mean ± SEM (n = 3 adult Id4-Egfp mice).(D) Quantification of Ki67 staining intensity in cells from (B) grouped by ID4-EGFPBright/SOX3low/RAR?low (SSCs) or ID4-EGFPBright/SOX3high/RAR?low (early progenitors). Data are mean ± SEM. Two-tailed Student’s t test (**p < 0.01).(E–G) WM-IIF of PLZF (green), SOX3 (red), Ki67 (white), and peanut agglutinin (PNA) in adult C57BL/6 mice (n = 4). Scale bar, 20 µm. Arrowhead, quiescent SSCs (PLZF+/SOX3-/Ki67-); arrow, activated SSCs (PLZF+/SOX3-/Ki67+).(H) Proportion of quiescent SSCs and activated SSCs in each stage from (E) and (F). Data are mean ± SEM. The proportion of Ki67+ SSCs was not significantly different according to ANOVA (p = 0.093).
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PLoS ONE
November 19 2014

Fig 3: SOX3 binding sites at enhancer regions.The overlap of SOX3 peaks with P300 binding sites identified from 11.5 dpc mouse; forebrain (A), midbrain (B), and limb (C), showing a high degree of overlap in the developing brain and not within the limb. Average phastCons score of the common peaks between SOX3 and P300 forebrain (D) and midbrain (E) binding sites.
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PLoS ONE
January 26 2012

Fig 4: Sox3 transgenic mice displayed CH. A,B: H&E stained coronal section of adult Sox3 transgenic mouse brain without (A) and with (B) overt hydrocephalus. Note the expanded lateral ventricles (LV), thinning of cerebral cortex (double-headed arrow) and hippocampal deformation (arrows) in the CH founder. C,D: Nr/+ adult mice showing dome-shaped cranium (arrow) due to overt CH (D) and normal cranial morphology (C). E,F: Nissl stained 18.5 dpc coronal brain sections of wild type (E) and Sr/+;Nr/+ (F) 18.5 dpc embryonic brains. Note the expansion of the lateral ventricles (LV) in the transgenic embryo (F). Scale bar: 2 mm (A–B) and 1 mm (E–F).
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Cell reports
February 16 2021

Fig 5: mTORC1 inhibition fails to drive activated SSC accumulation and early progenitor depletion in the absence of intercellular bridges(A and B) WM-IIF of PLZF (green, spermatogonia), RAR? (red, late progenitors), SOX3 (white, early/late progenitors) or KIT (white, differentiating spermatogonia), and Ki67 (blue, proliferation) in seminiferous tubules from P36 Tex14-/- mice. Arrowhead, quiescent SSCs (PLZF+/SOX3-/RAR?/Ki67-) or early progenitors (PLZF+/KIT-/RAR?-/Ki67-); arrow, activated SSCs (PLZF+/SOX3-/RAR?-/Ki67+) or early progenitors (PLZF+/KIT-/RAR?-/Ki67+); circle, early progenitors (PLZF+/SOX3+/RAR?-/Ki67+); asterisk, late progenitors (PLZF+/SOX3+ or KIT-/RAR?+/Ki67-). Scale bar, 20 µm.(C) Proportion of SSCs (KIT-/PLZFlow/SOX3low/RAR?low), early progenitors (KIT-/PLZFlow/SOX3high/RAR?low), and late progenitors (KIT-/PLZFhigh/SOX3high/RAR?high) in seminiferous tubule cells from control (n = 3) and rapamycin-treated (n = 3) Tex14-/- mice at P36 (see controls in Figure S3J). Data are mean ± SEM. (D) Ki67 staining intensity in SSCs (KIT-/PLZFlow/SOX3low/RAR?low), early progenitors (KIT-/PLZFlow/SOX3high/RAR?low), and late progenitors (KIT-/PLZFhigh/SOX3high/RAR?high) in seminiferous tubule cells from Tex14+/-, Tex14-/-, and rapamycin-treated Tex14-/- mice (n = 3 each) at P36 (see controls in Figure S3J). Data are mean ± SEM. Two-tailed Student’s t test (***p < 0.001).(E) Proportion of SSCs (KIT-/PLZFlow/SOX3low/RAR?low), early progenitors (KIT-/PLZFlow/SOX3high/RAR?low), and late progenitors (KIT-/PLZFhigh/SOX3high/RAR?high) in seminiferous tubule cells from adult control (n = 3) and rapamycin-treated (n = 3) Tex14-/- mice (see controls in Figure S3J). Data are mean ± SEM. Two-tailed Student’s t test (***p < 0.001).(F) Ki67 staining intensity in SSCs (KIT-/PLZFlow/SOX3low/RAR?low), early progenitors (KIT-/PLZFlow/SOX3high/RAR?low), and late progenitors (KIT-/PLZFhigh/SOX3high/RAR?high) in seminiferous tubule cells from adult (>60 days) control (n = 3) and rapamycin-treated (n = 3) Tex14-/- mice (see controls in Figure S3J. Data are mean ± SEM. Two-tailed Student’s t test (***p < 0.001).(G) Model positing that the transition between activated SSCs and clones of early progenitors is dependent upon mTORC1 activity.

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SOX3 Antibody

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The SOX3 Antibody from R&D Systems, a Bio-Techne Brand is a Goat Polyclonal antibody to SOX3, and SRY-box transcription factor 3. This antibody recognizes Homo Sapiens, and Human antigen. The SOX3 Antibody has been validated for the following applications: Immunocytochemistry, and Western Blot.

Product Specs
ItemSOX3 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF2569
NCBI Gene AliasesSOXB, PHPX, PHP, MRGH, GHDX
ApplicationsWestern Blot, Immunocytochemistry
Conjugate/TagUnconjugated
IsotypeIgG
TargetSOX3
HostGoat
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityHuman
NCBI Full Gene NameSRY-box transcription factor 3
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human SOX3, Met1-Leu230, Accession # P41225
ConcentrationLYOPH
PurityAntigen Affinity-purified
Brand/TrademarkR&D Systems, a Bio-Techne Brand
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects human SOX3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SOX2, rhSOX7, rhSOX10, and rhSOX17 is observed

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R&D Systems, a Bio-Techne Brand

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(1) Spermatogonial fate in mice with increased activin A bioactivity and testicular somatic cell tumoursFrontiers in Cell and Developmental BiologyJuly 26, 2023Penny A. F. Whiley, Benedict Nathaniel, Peter G. Stanton, Robin M. Hobbs, Kate L. Loveland,R&D Systems,1:250,NA GILZ,Rat,14–4033–82,Invitrogen,1:250,RRID:AB_1311225 LIN28A,Rabbit,#8641,Cell Signalling,1:500,RRID:AB_10997528 GCNA,Rat,ab82527,Abcam,1:500,RRID:AB_1659152 SOX3,Goat,AF2569,R&D Systems,1:250,RRID:AB_2239933 LAMININ,Rabbit,L9393,Sigma-Aldrich,1:200,RRID:AB_477163 CYP11A1,Rabbit,,gift

(2) Chromatin remodelers HELLS, WDHD1 and BAZ1A are dynamically expressed during mouse spermatogenesisReproductionJanuary 1, 2023Ram Prakash Yadav, Sini Leskinen, Lin Ma, Juho-Antti Mäkelä, Noora Kotaja(1:200, Millipore, ABD41), WDHD1 (1:200, A301-141A-T), GFRa1 (AF560, R&D Systems, 1:250) and SOX3 (AF2569, R&D Systems, 1:200) and DNMT3A (IMG-268A, Imgenex/Novus, 1:200). Following washes, the tubules were

(3) Single-cell transcriptomic landscapes of the otic neuronal lineage at multiple early embryonic agesCell reportsMarch 22, 2022Sun Y, Wang L, Zhu T, Wu B, Wang G, Luo Z, Li C, Wei W, Liu Z.

(4) An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogoniaCell reportsFebruary 16, 2021Suzuki S, McCarrey JR, Hermann BP.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies "Goat anti-SOX3 antibody (0.8 μg/mL); Figures 2, 3, 5, and S3–S5",R&D Systems,RRID: AB_2239933 Cat# AF2569 Goat anti-c-KIT antibody (4 μg/mL); Figures 5 and S3–S5,R&D Systems,RRID: AB_354750 Cat# AF1356 "Mouse

(5) The Nestin neural enhancer is essential for normal levels of endogenous Nestin in neuroprogenitors but is not required for embryo developmentPloS oneJanuary 1, 2021Thomson E, Dawson R, H'ng CH, Adikusuma F, Piltz S, Thomas PQ.and contrast using Adobe Photoshop v7 (Adobe Systems). Antibodies used were Anti-SOX3 (R&D Systems, AF2569, 1/200), Anti-Nestin (Abcam AB82375, 1/1000), Anti-CD31 (BD Pharmingen 550274, 1/100). Secondary antibodies,

(6) SOX3 promotes generation of committed spermatogonia in postnatal mouse testesScientific reportsApril 21, 2020McAninch D, Mäkelä JA, La HM, Hughes JN, Lovell-Badge R, Hobbs RM, Thomas PQ.described16,20. Antibodies Antibodies are detailed previously16,20,30,31,33, including αSOX3 (R&D Systems AF2569), αDNMT3A (Novus Biologicals 64B1446), αDNMT3B (Novus Biologicals 52A1018), α c-KIT (Cell Signaling Technologies

(7) DDX5 plays essential transcriptional and post-transcriptional roles in the maintenance and function of spermatogoniaNature communicationsMay 23, 2019Legrand JMD, Chan AL, La HM, Rossello FJ, Änkö ML, Fuller-Pace FV, Hobbs RM.R&D Systems, #AF1356); rabbit anti-ILF3 (1:500, Abcam, #ab92355); goat anti-SOX3 (1:250, R&D Systems, #AF2569); rabbit anti-VASA (1:250, Cell Signalling Technology, #8761); rabbit anti-SOX9 (1:1000, Millipore,

(8) GILZ-dependent modulation of mTORC1 regulates spermatogonial maintenanceDevelopment (Cambridge, England)September 18, 2018La HM, Chan AL, Legrand JMD, Rossello FJ, Gangemi CG, Papa A, Cheng Q, Morand EF, Hobbs RM.Primary antibodies were as follows: goat anti-PLZF (AF2944, 1:500), anti-GFRα1 (AF560, 1:250), anti-SOX3 (AF2569, 1:250), anti-c-KIT (AF1356, 1:250) and anti-E-cadherin (AF748, 1:250) (all R&D Systems); rabbit anti-phospho-RPS6

(9) Identification of dynamic undifferentiated cell states within the male germlineNature communicationsJuly 19, 2018La HM, Mäkelä JA, Chan AL, Rossello FJ, Nefzger CM, Legrand JMD, De Seram M, Polo JM, Hobbs RM.anti-mouse/rat PDX1 (AF2517, 1:250), anti-PLZF (AF2944, 1:500), anti-LIN28A (AF3757, 1:500) and anti-SOX3 (AF2569, 1:250) (R&D Systems), rabbit anti-mCherry (ab167453, 1:2000), anti-OCT4 (ab19857, 1:500), anti-SALL4

(10) An Eya1-Notch axis specifies bipotential epibranchial differentiation in mammalian craniofacial morphogenesiseLifeNovember 15, 2017Zhang H, Wang L, Wong EYM, Tsang SL, Xu PX, Lendahl U, Sham MH.immunostaining was performed using anti-Sox2 (1/500, GT15098-100, Neuromics, Minnesota, USA), anti-Sox3 (1/500, AF2569, R and D systems, Minnesota, USA), anti-Jagged1 (1/300, Ts1.15h, DSHB, Iowa, USA) and anti-E-cadherin

(11) Identification of Highly Conserved Putative Developmental Enhancers Bound by SOX3 in Neural Progenitors Using ChIP-SeqPLoS ONENovember 19, 2014Dale McAninch, Paul Thomaschromatin was immunoprecipitated by a goat polyclonal antibody raised against human SOX3 (R&D systems, AF2569). DNA was recovered by reversing crosslinks, and purified by PCR clean-up kit (QIAGEN). Three independent

(12) Congenital Hydrocephalus and Abnormal Subcommissural Organ Development in Sox3 Transgenic MicePLoS ONEJanuary 26, 2012Kristie Lee et al.as described previously [23]. Antibodies and dilutions were: goat anti-SOX3 (R&D systems cat. no.: AF2569, 1∶100), rabbit anti-EGFP (Abcam cat. no.: ab290, 1∶1000), rabbit anti-RF (kindly donated by A. Meiniel;

(13) Identification of SOX3 as an XX male sex reversal gene in mice and humansThe Journal of clinical investigationJanuary 1, 2011Sutton E, Hughes J, White S, Sekido R, Tan J, Arboleda V, Rogers N, Knower K, Rowley L, Eyre H, et al.

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3/1/2022Good SOX3 Antibody for Immunostaining James CarrollConstructed mice tissue immunostained for sox3. Signal works well, expression increased compared to WT control mice.

Cell reports

Cell reports

PLoS ONE

PLoS ONE

Cell reports

SOX3 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (13)

Reviews

SOX3 Antibody from R&D Systems, a Bio-Techne Brand

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